abstract：AIM:The aim of this study was to further characterize a bacterial culture (VUN 10,010) capable of benzo[a]pyrene cometabolism. METHODS AND RESULTS:The bacterial culture, previously characterized as a pure culture of Stenotrophomonas maltophilia (VUN 10,010), was found to also contain another bacterial species (Mycobac...

journal_title：Journal of applied microbiology

authors： Dandie CE,Thomas SM,Bentham RH,McClure NC

更新日期：2004-01-01 00:00:00

abstract：AIMS:The present work aimed to obtain bioinsecticide over-producing mutants through classical mutagenesis of vegetative cells of Bacillus thuringiensis (Bt) by using u.v. and nitrous acid, and to evidence the involvement of cell metabolism in delta-endotoxin synthesis. METHODS AND RESULTS:Vegetative cells of Bt were t...

journal_title：Journal of applied microbiology

authors： Ghribi D,Zouari N,Jaoua S

更新日期：2004-01-01 00:00:00

abstract：AIMS:To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnare. METHODS AND RESULTS:Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragm...

journal_title：Journal of applied microbiology

authors： Arias CR,Welker TL,Shoemaker CA,Abernathy JW,Klesius PH

更新日期：2004-01-01 00:00:00

abstract：AIMS:To establish the microbiological quality of cold ready-to-eat sliced meats and pâté from catering and retail premises, and investigate links hypothesized between foodborne Campylobacter infection and the consumption of cold sliced meats. METHODS AND RESULTS:A total of 4078 cold meat and pâté samples were collecte...

journal_title：Journal of applied microbiology

authors： Elson R,Burgess F,Little CL,Mitchell RT,Local Authorities Co-Ordinators of Regulatory Services and the Health Protection Agency.

更新日期：2004-01-01 00:00:00

abstract：AIM:Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. METHODS AND RESULTS:The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interac...

journal_title：Journal of applied microbiology

authors： Thage BV,Rattray FP,Laustsen MW,Ardö Y,Barkholt V,Houlberg U

更新日期：2004-01-01 00:00:00

abstract：AIMS:To determine the mechanisms of Bacillus subtilis spore killing by and resistance to the general biological decontamination agents, Decon and Oxone. METHODS AND RESULTS:Spores of B. subtilis treated with Decon or Oxone did not accumulate DNA damage and were not mutagenized. Spore killing by these agents was increa...

journal_title：Journal of applied microbiology

authors： Young SB,Setlow P

更新日期：2004-01-01 00:00:00

abstract：AIMS:To identify and examine the diversity of predominant lactic acid bacteria (LAB) in koko and koko sour water (KSW) from different Ghanaian production sites with regard to pattern of fermentation (API 50 CHL), genotype, antimicrobial activity, and resistance to low pH and bile salts. METHODS AND RESULTS:In total 21...

journal_title：Journal of applied microbiology

authors： Lei V,Jakobsen M

更新日期：2004-01-01 00:00:00

abstract：AIMS:To isolate and identify endophytic nitrogen-fixing bacteria in sugarcane growing in Cuba without chemical fertilizers. METHODS AND RESULTS:Two N2-fixing isolates, 9C and T2, were obtained from surface-sterilized stems and roots, respectively, of sugarcane variety ML3-18. Both isolates showed acetylene reduction a...

journal_title：Journal of applied microbiology

authors： Loiret FG,Ortega E,Kleiner D,Ortega-Rodés P,Rodés R,Dong Z

更新日期：2004-01-01 00:00:00

abstract：AIMS:To investigate the influence of micro-organisms associated with copper corrosion on 'blue water' corrosion in drinking water. METHODS AND RESULTS:Laboratory rigs comprising of polycarbonate containers attached to annealed copper plumbing tubes were filled with Melbourne drinking water and sterilized by autoclavin...

journal_title：Journal of applied microbiology

authors： Critchley MM,Pasetto R,O'Halloran RJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:The purpose of this study was to comprehensively compare the response of nine biosensors capable of being induced by Hg. Induction by Hg was based upon the insertion of merR, merB, zntA and zntR promoter genes. LuxCDABE or lucFF reporter genes expressed luminescence, and host organisms were Escherichia coli, Vibri...

journal_title：Journal of applied microbiology

authors： Harkins M,Porter AJ,Paton GI

更新日期：2004-01-01 00:00:00

abstract：AIMS:To determine the effects of herbal essential oils on Trichophyton spp. growth and to evaluate the effects of Pelargonium graveolens oil and its main components citronellol and geraniol combined with ketoconazole against Trichophyton spp. METHODS AND RESULTS:Growth inhibition of six Trichophyton spp. by herbal ess...

journal_title：Journal of applied microbiology

authors： Shin S,Lim S

更新日期：2004-01-01 00:00:00

abstract：AIMS:Monitoring of microbial changes during and after application of various disinfection treatments in a model domestic water system. METHODS AND RESULTS:A pilot-scale domestic water system consisting of seven galvanized steel re-circulation loops and copper dead legs was constructed. Culture techniques, confocal las...

journal_title：Journal of applied microbiology

authors： Thomas V,Bouchez T,Nicolas V,Robert S,Loret JF,Lévi Y

更新日期：2004-01-01 00:00:00

abstract：AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...

journal_title：Journal of applied microbiology

authors： Bejaoui H,Mathieu F,Taillandier P,Lebrihi A

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (COD) of the spent wash. ...

journal_title：Journal of applied microbiology

authors： Ghosh M,Verma SC,Mengoni A,Tripathi AK

更新日期：2004-01-01 00:00:00

abstract：AIMS:To determine the prevalence of enterotoxin-producing Staphylococcus intermedius in dogs and pigeons. METHODS AND RESULTS:A total of 106 S. intermedius isolates from 44 dogs and 62 pigeons were tested for the production of enterotoxins A, B, C and D by reverse passive latex agglutination (RPLA) and for sec-canine ...

journal_title：Journal of applied microbiology

authors： Futagawa-Saito K,Suzuki M,Ohsawa M,Ohshima S,Sakurai N,Ba-Thein W,Fukuyasu T

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aims of this study were to develop a sensitive and more rapid detection of Propionibacterium acidipropionici DH42 in silage and rumen fluid samples, and to explore its 16S rRNA sequence-based phylogeny. METHODS AND RESULTS:Nested polymerase chain reaction (PCR) was used with DH42-specific primers dhb1 and dhb...

journal_title：Journal of applied microbiology

authors： Romanov MN,Bato RV,Yokoyama MT,Rust SR

更新日期：2004-01-01 00:00:00

abstract：AIMS:The transfer of tetO gene conferring resistance to tetracycline was studied between Campylobacter jejuni strains, in the digestive tract of chickens. METHODS AND RESULTS:In vitro conjugation experiments were first performed in order to select donor/recipient couples for further in vivo assay. Then, chickens were ...

journal_title：Journal of applied microbiology

authors： Avrain L,Vernozy-Rozand C,Kempf I

更新日期：2004-01-01 00:00:00

abstract：AIMS:Point-of-use drinking water disinfection with sodium hypochlorite has been shown to improve water quality and reduce diarrhoeal disease. However, the chlorine demand of highly turbid water may render sodium hypochlorite less effective. METHODS AND RESULTS:We evaluated a novel combined flocculant-disinfectant poin...

journal_title：Journal of applied microbiology

authors： Crump JA,Okoth GO,Slutsker L,Ogaja DO,Keswick BH,Luby SP

更新日期：2004-01-01 00:00:00

abstract：AIMS:Isolates of Candida valida, Rhodotorula glutinis and Trichosporon asahii from the rhizosphere of sugar beet in Egypt were examined for their ability to colonize roots, to promote plant growth and to protect sugar beet from Rhizoctonia solani AG-2-2 diseases, under glasshouse conditions. METHODS AND RESULTS:Root c...

journal_title：Journal of applied microbiology

authors： El-Tarabily KA

更新日期：2004-01-01 00:00:00

abstract：AIMS:Ascospores of Talaromyces macrosporus are constitutively dormant and germinate after a strong external shock, classically a heat treatment. This fungus is used as a model system to study heat resistance leading to food spoilage after pasteurization. This study evaluates the effect of high pressure on the germinati...

journal_title：Journal of applied microbiology

authors： Dijksterhuis J,Teunissen PG

更新日期：2004-01-01 00:00:00

abstract：AIMS:To determine whether the beer-spoilage ability is an intrinsic character of Lactobacillus paracollinoides and identify a genetic marker for differentiating the beer-spoilage ability of strains belonging to this species. METHODS AND RESULTS:The ribotype of a nonspoilage strain, Lact. brevis ATCC8291, was found to ...

journal_title：Journal of applied microbiology

authors： Suzuki K,Ozaki K,Yamashita H

更新日期：2004-01-01 00:00:00

abstract：AIMS:Enumeration of resistant bacteria in ultra-high temperature (UHT) treated milk; morphological characterization and phenotyping of resistant strains by traditional and nontraditional methods and their identification by molecular biology. METHODS AND RESULTS:Modified standard plate count agar (PCA) and modified bra...

journal_title：Journal of applied microbiology

authors： Montanari G,Borsari A,Chiavari C,Ferri G,Zambonelli C,Grazia L

更新日期：2004-01-01 00:00:00

abstract：AIMS:To characterize the non-spoilage variant obtained from beer-spoilage Lactobacillus brevis ABBC45C and to identify a potential genetic marker capable of discriminating beer-spoilage L. brevis strains from non-spoilers. METHODS AND RESULTS:A non-spoilage variant was obtained from beer-spoilage L. brevis ABBC45C by ...

journal_title：Journal of applied microbiology

authors： Suzuki K,Koyanagi M,Yamashita H

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aims of this study were to characterize the molecular variations in the quinolone resistance-determining region (QRDR) of gyrA among quinolone-resistant and -susceptible Campylobacter jejuni isolates originating from foods of animal origin and human infections and to evaluate the suitability of the single-stra...

journal_title：Journal of applied microbiology

authors： Beckmann L,Müller M,Luber P,Schrader C,Bartelt E,Klein G

更新日期：2004-01-01 00:00:00

abstract：AIMS:To develop a method for studying the microbial spoilage of water-in-oil emulsions and to use this to investigate (i) the intrinsic stability of water-in-oil formulations and (ii) Pseudomonas aeruginosa SP1-induced spoilage of a proprietary emulsion. METHODS AND RESULTS:Aliquots of test emulsion were placed into w...

journal_title：Journal of applied microbiology

authors： O'May GA,Allison DG,Gilbert P

更新日期：2004-01-01 00:00:00

abstract：AIM:To study the microbiology of intensive, in-vessel biodegradation of a mixture of sewage sludge and vegetable food waste. METHODS AND RESULTS:The biodegradation was performed in a closed reactor with the addition of a starter culture of Bacillus thermoamylovorans SW25 under conditions of controlled aeration, stirri...

journal_title：Journal of applied microbiology

authors： Ivanov VN,Wang JY,Stabnikova OV,Tay ST,Tay JH

更新日期：2004-01-01 00:00:00

abstract：AIMS:The effect of five essential oils (oregano, cinnamon, lemongrass, clove and palmarose) on growth rate, zearalenone (ZEA) and deoxynivalenol (DON) production by Fusarium graminearum strains was assessed. METHODS AND RESULTS:The influence of the essential oils was tested on irradiated maize at two concentrations (5...

journal_title：Journal of applied microbiology

authors： Velluti A,Sanchis V,Ramos AJ,Turon C,Marín S

更新日期：2004-01-01 00:00:00

abstract：AIMS:We have been for a species of thermophilic bacteria that can effectively decompose collagen and collagen peptides that tend to be hard-to-degrade proteins because of their high content of proline residues. This study focused upon the enzymatic degradation of prolyl peptides by thermophilic bacteria. METHODS AND R...

journal_title：Journal of applied microbiology

authors： Murai A,Tsujimoto Y,Matsui H,Watanabe K

更新日期：2004-01-01 00:00:00

abstract：AIM:Calibration of impedance measurement was performed vs the Association Françoise de Normalisation (AFNOR) MPN method with a view to rapid enumeration of Escherichia coli in live marine bivalve molluscs. METHODS AND RESULTS:Linear regression models between log10 MPN and detection time (DT) were adjusted for several ...

journal_title：Journal of applied microbiology

authors： Dupont J,Dumont F,Menanteau C,Pommepuy M

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aim of this study was to determine the survival and growth characteristics of Salmonella enterica in sound and chill-injured tomatoes as influenced by co-infection with proteolytic moulds. METHODS AND RESULTS:Sound (not chill injured) raw, ripe tomatoes (Lycopersicon esculentum Mill. 'Roma') were inoculated w...

journal_title：Journal of applied microbiology

authors： Wade WN,Beuchat LR

更新日期：2003-01-01 00:00:00

abstract：AIMS:Production of isomaltulose by newly isolated Klebsiella sp. LX3. METHODS AND RESULTS:The bacterial isolate LX3, which transforms sucrose to isomaltulose and trehalulose, has been isolated from a soil sample in Singapore. Morphological and biochemical analysis, as well as 16s rRNA sequence demonstrated that the is...

journal_title：Journal of applied microbiology

authors： Li X,Zhao C,An Q,Zhang D

更新日期：2003-01-01 00:00:00

abstract：AIMS:To assess the presence of virulence genes in environmental and foodborne Escherichia coli isolates using the TaqMan PCR system. METHODS AND RESULTS:Three TaqMan pathogen detection kits called O157:H7, StxI and StxII were used to investigate the presence of virulence genes in Escherichia coli isolates. All 54 food...

journal_title：Journal of applied microbiology

authors： Davis KC,Nakatsu CH,Turco R,Weagant SD,Bhunia AK

更新日期：2003-01-01 00:00:00

abstract：AIMS:A fluorescent in situ hybridization (FISH) technique using an Enterobacteriaceae-specific probe (probe D) to target 16S rRNA was improved in order to enumerate, within a single working day, Enterobacteriaceae present in food and environmental water samples. METHODS AND RESULTS:In order to minimize the time requir...

journal_title：Journal of applied microbiology

authors： Ootsubo M,Shimizu T,Tanaka R,Sawabe T,Tajima K,Ezura Y

更新日期：2003-01-01 00:00:00

abstract：AIMS:To determine the ability of 149 clinical isolates of Staphylococcus aureus from burns, other wounds and environmental isolates to adhere to immobilized proteins. METHODS AND RESULTS:The ability to bind to immobilized fibrinogen, fibronectin, laminin, collagen, IgG and lysozyme was studied using a microtitre plate...

journal_title：Journal of applied microbiology

authors： Elgalai I,Foster HA

更新日期：2003-01-01 00:00:00

abstract：AIMS:To examine associations between growth rate within bacterial populations and survival patterns following treatment with antimicrobial agents. METHODS AND RESULTS:Time survival data were generated for the inactivation of Escherichia coli populations, grown as batch and continuous cultures, exposed to ciprofloxacin...

journal_title：Journal of applied microbiology

authors： Sufya N,Allison DG,Gilbert P

更新日期：2003-01-01 00:00:00

abstract：AIMS:The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. METHODS AND RESULTS:Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must...

journal_title：Journal of applied microbiology

authors： Larena I,De Cal A,Liñán M,Melgarejo P

更新日期：2003-01-01 00:00:00

abstract：AIMS:To establish a laboratory model to compare the effectiveness of detergent-based disinfection procedures for reducing cross-contamination risks during handling of contaminated chicken. METHODS AND RESULTS:During handling of chickens, artificially contaminated with Salmonella enteritidis PT4, the organism was widel...

journal_title：Journal of applied microbiology

authors： Barker J,Naeeni M,Bloomfield SF

更新日期：2003-01-01 00:00:00

abstract：AIMS:To apply a denaturing gradient gel electrophoretic (DGGE) method to quantify and profile individual strains during a mixed culture fermentation. METHODS AND RESULTS:DNA was extracted during the culture of lactic acid bacteria (LAB) and amplified in a multiple competitive PCR (cPCR) using general primers targeting...

journal_title：Journal of applied microbiology

authors： Pintado J,Guyot JP,Ampe F

更新日期：2003-01-01 00:00:00

abstract：AIMS:Monitoring for Salmonella in slaughter pigs is important to enable targeted control measures to be applied on problem farms and at the abattoir. The aim of this study was to determine whether pooled serum and meat juice could be used to identify finishing pig herds with a high prevalence of infection. METHODS AND...

journal_title：Journal of applied microbiology

authors： Davies RH,Heath PJ,Coxon SM,Sayers AR

更新日期：2003-01-01 00:00:00

abstract：AIMS:Intraspecific differentiation and characterization of Vibrio alginolyticus strains isolated from cultured fish in Spain. MATERIALS AND RESULTS:Thirty-four Vibrio alginolyticus strains isolated from cultured fish were intraspecifically characterized on the basis of biochemical and exoenzymatic patterns, outer memb...

journal_title：Journal of applied microbiology

authors： Zorrilla I,Moriñigo MA,Castro D,Balebona MC,Borrego JJ

更新日期：2003-01-01 00:00:00